Dextran sulfate stabilized silver nanoparticle: Next generation efficient therapy for cancer

Abstract

Objective: Synthesize silver nanoparticles using a green synthesis approach and encapsulate silver nanoparticles with a naturally occurring polymer, particularly of an-hydroglucose type, like dextran sulfate sodium salt and to study its anticancer activity. Methods: Green synthesis approach is been employed in the synthesis of silver nanoparticles using Psidium guajava leaf extract. The nanoparticles were then encapsulated with dextran sulfate biopolymer and the nanoparticles were subjected to different characterization techniques. The structure of the synthesized nanoparticles was analyzed using X-ray diffraction analysis, the presence of different functional groups was analyzed by FTIR studies. Size and morphology of the prepared nanoparticles were investigated using FESEM analysis. Anticancer activity of the synthesized nanoparticles was tested against the MCF-cell line. Results: The XRD analysis shows the crystalline nature of the synthesized nanoparticles. The stretching and vibrating modes of different functional groups were confirmed by FTIR result. The SEM image confirmed the presence of spherical shaped nanoparticles and the TEM image confirmed the average size of the particles to be around 24 nm. The Ag-DS NPs showed 91% cell inhibition for the concentration of 100 μg/ml, indicating the cytotoxicity of the nanoparticles against MCF-7 cell line. Conclusion: Dextran sulfate stabilized silver nanoparticles show potent anticancer activity against MCF-7 cell line.