Partial restoration of B cell development in Jak-3-/- mice achieved by co-expression of IgH and Eμ-myc transgenes

Abstract

Jak-3 is a non-receptor tyrosine kinase that plays an important role in coordinating signals received through a wide range of cytokine receptors, including the IL-7 receptor (IL-7R). Jak-3-deficient mice have a profound block in B cell development at the pro-to-pre-B cell transition and have very few peripheral B cells. This block has been postulated to reflect the inability of Jak-3-/- pro-B cells to respond to IL-7. Here we demonstrate that B cell development can be partially restored in Jak-3-deficient mice when they are bred to mice carrying both a rearranged Ig heavy chain (IgH/Igμ) transgene and a c-myc transgene expressed in the B cell lineage. Jak-3-/-mice expressing both of these transgenes exhibit significant increases in the number of B cells in the bone marrow and, to a lesser extent, in the spleen. However, very few rescued B cells were detectable in mice greater than 4 months of age. To determine whether resident hyperactivated Jak-3-/- peripheral T cells are responsible for the elimination of the rescued B cells in older mice, we bred IgH transgenic (Igμ Tg)/myc Tg/Jak-3-/- mice to T cell-deficient (TCRα-/-) mice. Data from these experiments suggest that the paucity of B cells in older Jak-3-/- mice is largely attributable to the lack of Jak-3 in the B cells themselves. Thus, Jak-3 seems to play several important roles in B cells: during development, to enable cell division, Ig gene rearrangement and cell differentiation, and in mature cells, to promote B cell survival in the periphery.