Characterization of polyphosphate glucokinase SCO5059 from streptomyces coelicolor A3(2)

Abstract

SCO5059, encoded in Streptomyces coelicolor A3(2), was identified as a polyphosphate glucokinase. The Km values of SCO5059 for glucose and polyphosphate (poly(P)6) were estimated to be 12 and 4μM, respectively, and the kcat value was 0.3 s-1 at pH 7.7 at 28 °C. SCO5059 homologs are highly conserved among Streptomyces, and can work as polyphosphate glucokinase as well.