Production of biobuthanol from various lignocellulose waste with Clostridium acetobutylicum bacteria using ABE (acetone-buthanol-ethanol) fermentation

Abstract

Biobuthanol is produced by fermentation of lignocellulose waste from Clostridium acetobutylicum by the ABE (Acetone-Butanol-Ethanol) fermentation method. In this research on biobuthanol production, variations in the type and composition of lignocelluloses raw materials (rice husk and corn husk) were carried out. The steps of this research include delignification, hydrolysis, fermentation, and yield analysis. The results found in this study are that in the comparison of types and composition mixture of raw materials, the highest production of biobuthanol is achieved in samples with a variation of the composition of the raw material mixture of 25% rice husk and 75% corn husk, this is due to the discovery of the highest levels of cellulose with the least lignin in the sample, so that the hydrolysis process can take place quickly and produce more glucose as the main substrate in biobuthanol fermentation.