Characterization of the causal agent of the deformation of the fruits of the grape (vitis vinifera l.) var. Red globe, in la unión, valle del cauca, colombia

Abstract

In recent years, a disease characterized by the necrosis of the grape berries (Vitis viinifera L.) has appeared on the Red Globe variety cultivated in vineyards in the municipality of La Unión, Valle del Cauca, Colombia, resulting in loss of yield and quality. To identify the etiology of the disease, fruits showing the typical symptoms of discoloration and subsequent necrosis were collected and processed. With high frecuency was found a fungus that was subjected to pathogenicity tests and morphological characterization by light and electron microscopy (ESEM). Koch´s postulates were fulfilled by the inoculation of a conidial suspension of 1x106 conidia per milliliter of water on healthy berries of the same variety. After a week, symptoms similar to those observed in the field began to be observed. The search for similitudes with BLAST showed that the sequences of ITS had a 100% identity with Colletotrichum aenigma and C. siamense belonging to the complex of species of Colletotrichum gloeosporioides, which provides useful information to understand the disease of the grape berries and to design management strategies to control it.