PCR-RFLP to detect codon 248 mutation in exon 7 of p53 tumor suppressor gene

Abstract

Individual genome DNA was extracted fast from oral swab and followed up with PCR specific for codon 248 of p53 tumor suppressor gene. MspI restriction mapping showed the G-C mutation in codon 248, which closely relates to cancer susceptibility. Students learn the concepts, detection techniques, and research significance of point mutations or single nucleotide polymorphisms in individual genomes. © 2009 by The International Union of Biochemistry and Molecular Biology.