Ponto-Caspian amphipod co-location with zebra mussel beds (Dreissena polymorpha) is influenced by substrate size and population source

Abstract

The global spread of non-native species is leading to an increasing frequency of multiple co-occurring non-native species. We examined the co-occurrence of the bivalve mollusc Dreissena polymorpha (zebra mussel) with three Ponto-Caspian amphipods (Dikerogammarus villosus, Dikerogammarus haemobaphes, and Chelicorophium curvispinum) across England and Wales in association with in-situ substrate size. For all three amphipod species, substrate grain size where amphipods co-occurred with D. polymorpha was significantly finer than when recorded in isolation. Subsequently, we confirmed this via aquarium experiments. We examined the occurrence of D. villosus with D. polymorpha when present with cobbles, gravel, or sand from three population sources (co-location with abundant D. polymorpha populations, co-location with low populations, and naïve). Experiments demonstrated that D. villosus actively sought shelter on or near D. polymorpha, with their co-location being significantly more prevalent in finer grained substrates (sand > gravel > cobble). The strength of this co-location differed by population source, with those co-located with high D. polymorpha densities demonstrating a greater association. Our analyses and experiments indicate that D. polymorpha may facilitate Ponto-Caspian amphipod establishment in otherwise suboptimal locations, whereby mussel shells provide favourable structural habitat for the amphipods, analogous to the presence of coarse-grained benthic sediment.