Abstract
The metal-responsive smt operator/promoter region of Synechococcus PCC7942 was fused to the luxCDABE genes of Vibrio fischeri. Plasmid DNA (pJLE23) carrying this fusion conferred metal ion-inducible luminescence to transformed cyanobacteria. Synechococcus PCC7942 (pJLE23) was sensitive to ZnCl2 concentrations within a range of 0.5-4 μM as demonstrated by induction of luminescence. Trace levels of CuSO4 and CdCl2 were also detected.
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Erbe, J. L., Adams, A. C., Taylor, K. B., & Hall, L. M. (1996). Cyanobacteria carrying an smt-lux transcriptional fusion as biosensors for the detection of heavy metal cations. Journal of Industrial Microbiology, 17(2), 80–83. https://doi.org/10.1007/BF01570047
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