Methods of Plant Transformation- A Review

Abstract

Plant transformation is now a core research tool in plant biology and a practical tool for transgenic plant development. There are many verified methods for stable introduction of novel genes into the nuclear genomes of diverse plant species. As a result, gene transfer and regeneration of transgenic plants are no longer the factors limiting the development and application of practical transformation systems for many plant species. However, the desire for higher transformation efficiency has stimulated work on not only improving various existing methods but also in inventing novel methods. The most published techniques for gene transfer into plant cells were dismissed as either disproven or impractical for use in routine production of transgenic plants. In many laboratories, virtually all the transformation work relies on particle bombardment with DNA coated microprojectiles or Agrobacterium mediated transformation for gene transfer to produce transgenic plants from a range of plant species.