Identification of phytotoxic substances in soils following winter injury of grasses as estimated by a bioassay

Abstract

Under northern conditions, winter survival of grass species for hay production is quite uncertain because of winter stresses to plants. Damage to plants may be caused by variability in snow cover, low temperatures, ice encasement and pathogens. Compared to renovation by ploughing, direct drilling without ploughing has some beneficial aspects and may be an alternative method for renovating hay fields. However, successful establishment of grasses without ploughing has been difficult to achieve both in scientific studies and under practical conditions. The objective of this investigation was to study whether a dead grass sod, killed by different winter injuries, may leak phytotoxins into the soil, thus causing the poor results observed by direct drilling in hayfields. Experiments, including soil water extraction and the use of bioassays, were conducted in growth chambers, to study the effects of the winter stresses frost, ice encasement, and snow mould on the accumulation of potentially phytotoxic biochemical compounds in the soil. Snow mould did not kill the grasses in the experiments and no phytotoxicity was measured. Although both ice encasement and low temperatures killed the grasses, only ice encasement caused phytotoxicity. The present investigation shows that the occurrence of phytotoxic substances, especially butyrate, after ice encasement may be a cause of poor establishment of direct-drilled grass plants after winter injuries. In serious cases of ice encasement this may justify a 1-2 wk delay of sowing, which in turn may result in reduced soil water for germination and may cause increased competition from other species.