Antimicrobial Activity of Aristolochia tagala Cham. Centella asiatica Linn. Houttuynia cordata Thunb. on Multi- drug Resistant Clinical Isolates

Abstract

The objective of this study is to evaluation of antimicrobial activity of Aristolochia tagala Cham. (AT), Centella asiatica Linn. (CA), Houttuynia cordata Thunb (HC) on multi-drug resistant bacteria isolated from clinical samples. Alcoholic extracts of these plants carried out by adopting Kirby-Bauer disc diffusion method against standard strains (ATCC) and resistant clinical isolates of Staphyllococcus aureus, Enterococcus faecalis and Staphyllococcus aureus. Piperacillin, ampicillin, amikacin, ciprofloxacin, and cefotaxime served as positive controls in the standard test and nitrofurantoin, teicoplanin, linezolid, and vancomycin were used in the testing of the resistant isolates. 100 mg/mL of the plant extracts were used as the test extracts taking 2.5 % DMSO as a negative control. From the zone of inhibition (mm) recorded, AT demonstrated higher antimicrobial efficacy against Enterococcus and S. aureus. Another significant finding is HC showed considerable effectiveness against E.Coli. However, methanolic extract of CA showed only moderate antimicrobial activity. Good antibacterial activity was seen with AT alcoholic extracts for Enterococcus and S. aureus resistant strains in clinical isolates. Antibacterial activity of HC against E. coli isolates also holds promising results.