Stimulatory constituents of eclipta prostrata on mouse osteoblast differentiation

Abstract

One flavonoid, diosmetin (1), and two isoflavonoids, 3′- hydroxybiochanin A (2) and 3′-O-methylorobol (3), were isolated from the methanol extract of Eclipta prostrata L. by a bioactivity-guided fractionation technique using primary cultures of mouse osteoblasts as an in vitro assay system. All three compounds significantly increased osteoblast differentiation as assessed by the alkaline phosphatase activity. Copyright © 2008 John Wiley & Sons, Ltd.