Characterization of HIV-1 RNA forms in the plasma of patients undergoing successful HAART

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Abstract

An assay to characterize plasma human immunodeficiency virus 1 (HIV-1) sequences for patients with low viral loads was developed by combining the selective binding of anti-CD44 MicroBeads with a nested RT-PCR targeting the env C2V4 region. Sequences were obtained from 10 of 20 HIV+ patients who had viral loads below 48 copies/ml. Sequences derived from plasma were compared to those from CD14+ CD16 +monocytes and CD4+ T cells. The plasma sequences were most closely related to those amplified from monocytes, suggesting that during successful antiretroviral therapy, the predominant plasma virus originates from myeloid cells. By characterizing HIV-1 RNA sequences from 8 ml of plasma while avoiding multiple steps, which can lead to contamination and deterioration, this method can help elucidate the viral forms in patients with therapeutically suppressed HIV-1. Understanding the source of residual viremia is crucial in developing approaches for viral eradication. © 2010 The Author(s).

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Lopez, C. A., Vazquez, M., Hill, M. D., Del C. Colon, M., Porrata-Doria, T., Johnston, I. C. D., & Lorenzo, E. (2010). Characterization of HIV-1 RNA forms in the plasma of patients undergoing successful HAART. Archives of Virology, 155(6), 895–903. https://doi.org/10.1007/s00705-010-0659-3

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