Komparasi Kualitas Semen Segar dan Beku pada Sapi Pejantan Limousin di Teaching Farm Fakultas Kedokteran Hewan Universitas Airlangga

  • Achmad A
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Abstract

Salah satu cara untuk mengurangi nilai impor daging sapi secara nasional yaitu dengan program peningkatan jumlah anakan sapi potong pada skala peternak rakyat, melalui peningkatan efisiensi reproduksi sapi menggunakan teknologi inseminasi buatan. Tujuan penelitian ini adalah untuk mengetahui perbedaan kualitas semen segar dan semen beku sapi pejantan Limousin. Metode penelitian menggunakan eksploratif laboratoris. Hasil pengamatan makroskopis menunjukkan bahwa nilai volume semen segar adalah 4,4-7,2 ml, sementara hasil pengamatan warna pada semen segar maupun semen beku semuanya berwarna putih susu. Konsistensi pada semen segar termasuk kental, sedangkan pada semen beku tergolong cair. Pengukuran pH pada semen segar dan beku menunjukkan hasil 6,7. Sementara itu, hasil pengukuran motilitas semen segar diperoleh nilai 77% dan pada semen beku menunjukkan hasil 41,5%. Abnormalitas semen segar menunjukkan nilai 1,30%, sedangkan pada semen beku diperoleh hasil 0,00%. Hasil pemeriksaan konsentrasi spermatozoa pada semen segar sebanyak 1.426(106)/ml, sedangkan pada semen beku sebesar 26,6(106)/ml. Hasil evaluasi mikroskopis menunjukkan bahwa terdapat lima variabel yang berbeda nyata (p<0,05) yaitu volume, kekentalan, motilitas, abnormalitas, dan konsentrasi, serta terdapat dua variabel yang menunjukkan tidak beda nyata (p>0,05) yaitu warna dan pH. Disimpulkan bahwa terdapat perbedaan nyata pada lima variabel yang diukur tetapi perbedaan tersebut masih dalam batas ambang yang wajar sesuai standar baku mutu kualitas yang diterbitkan oleh Badan Standarisasi Nasional Tahun 2021.One way to reduce the value of beef imports nationally is to conduct a program to increase the number of beef cattle breeders at the smallholder scale by increasing the efficiency of cattle reproduction through artificial insemination technology. The purpose of the study was to determine the difference in the quality of fresh semen and frozen semen of Limousin bulls. The research method used exploratory laboratory. The results of macroscopic observations showed that the value of fresh semen volume was 4.4-7.2 ml., the results of color observations on fresh and frozen semen were all milky white. The consistency of fresh semen is thick while frozen semen is liquid. Measurement of pH in fresh and frozen semen showed a result of 6.7, while the results of fresh semen motility measurements obtained a value of 77% and in frozen semen showed a result of 41.5%. Abnormality of fresh semen showed a value of 1.30% while in frozen semen the result was 0.00%. The results of the examination of spermatozoa concentration in fresh semen were 1,426(106)/ml, while in frozen semen it was 26.6(106)/ml. The results of microscopic evaluation showed that there were five variables that were significantly different (p<0.05), namely volume, viscosity, motility, abnormality, and concentration, and there were two variables that showed no significant difference (p>0.05), namely color and pH. It was concluded that there are significant differences in the five variables measured but these differences are still within reasonable threshold limits according to the quality standards published by the National Standardization Agency in 2021.

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APA

Achmad, A. R. (2025). Komparasi Kualitas Semen Segar dan Beku pada Sapi Pejantan Limousin di Teaching Farm Fakultas Kedokteran Hewan Universitas Airlangga. MANILKARA: Journal of Bioscience, 3(2), 76–88. https://doi.org/10.33830/manilkara.v3i2.11163.2025

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