Isolation and Identification of Biologically Active Components from the Honey Bee Venom Apis Mellifera L. Caucasica

Abstract

The article presents experimental data on the separation and identification of biologically active components of the venom of the honey bee Apis mellifera L. Caucasica. By gel chromatography on a column with Servacel-52 eluting with a linear gradient of NaCl concentration from 0.01 to 1M, followed by spectrophotometric measurement of the unit of optical density at an absorption wavelength of =280nm on a Hitachi-557 spectrophotometer from the honey bee venom, biologically active components of zootoxin with molecular weights of 12000, 14600 and 22000D. A polypeptide with Mg~12000D is a toxic component identified as mellitine, a polypeptide with Mg~14600 is an enzyme of phospholipase A2, and a polypeptide with Mg~22000D is a glycoprotein.