Single antigen flow beads for identification of human leukocyte antigen antibody specificities in hypersensitized patients with chronic renal failure

Abstract

Aims of this study were to identify class I and class II antibodies in highly sensitized patients by flow cytometry single antigen bead (FC-SAB) assay and to evaluate according to donor HLA type in order to increase their kidney transplantation chance. Material and methods: We analyzed 60 hypersensitive patients of 351 individuals, who applied to our laboratory for PRA test in November 2013-December 2014. Flow cytometric PRA screening and single antigen bead commercial kits were used for these analyses. Results: In our study group, 19 (31.7%) of these patients were male while 41 (68.3%) patients were female. The most common acceptable antigens were A∗02 (10.11%), HLA-A∗23 (10.11%), HLA-B∗38 (8.79%) and HLA-DRB 1∗03 (7.83%) in hypersensitive patients. The highest antibody reactivity on SAB was observed against HLA-A∗25, HLA-B∗45, HLA-DRB 1∗04 and HLA-DRB 1∗08 antigens. Conclusions: The determination of these acceptable and unacceptable antigens may increase their transplantation chance. Pre-transplant HLA antibody identifications provide prognostic information with respect to the determination of patients who are at increased risk of graft loss.