The impact of cAMP on ROS-balance in human and mammalian cell cultures was studied. cAMP reduced accumulation of ROS induced by serum-limitation, under conditions in which there was no significant change in the activity of scavenger systems. This effect was associated with cAMP-dependent activation of the NADH-ubiquinone oxidoreductase activity of complex I. In fibroblasts from a patient a genetic defect in the 75 kDa FeS-protein subunit of complex I resulted in inhibition of the activity of the complex and enhanced ROS production, which were reversed by cAMP. A missense genetic defect in the NDUFS4 subunit, putative substrate of PKA, suppressed, on the other hand, the activity of the complex and prevented ROS production. © 2006 Federation of European Biochemical Societies.
Piccoli, C., Scacco, S., Bellomo, F., Signorile, A., Iuso, A., Boffoli, D., … Papa, S. (2006). cAMP controls oxygen metabolism in mammalian cells. FEBS Letters, 580(18), 4539–4543. https://doi.org/10.1016/j.febslet.2006.06.085