We determined the carbohydrate structures of contact site A from Dictyostelium discoideum. The carbohydrate moieties of contact site A were released by hydrazinolysis. Fractionation of the deacidified oligosaccharide mixture by Bio-Gel P-4 column chromatography revealed that it was composed of four major oligosaccharides. Their respective structures were determined by sequential exoglycosidase digestion. It is known that contact site A consists of two kinds of carbohydrates, I and II. Taking together the previous and the present results, it was deduced that carbohydrate I comprises N-linked oligosaccharides and carbohydrate II O-linked ones. Furthermore, the relative molar contents of GalNAc and GlcNAc in reducing terminal suggested that contact site A contains 67% of N-linked and 33% of O-linked oligosaccharides. © 1993.
Yoshida, M., Matsui, T., Fuse, G., & Ouchi, S. (1993). Carbohydrate structures of the cell adhesion molecule, contact site A, from Dictyostelium discoideum. FEBS Letters, 318(3), 305–309. https://doi.org/10.1016/0014-5793(93)80534-2