Isolation and identification of a marine actinomycete strain and its control efficacy against citrus green and blue moulds

Abstract

The most serious citrus postharvest diseases are citrus green mould caused by Penicillium digitatum and citrus blue mould caused by Penicillium italicum. Marine microorganisms have become a new measure in the control of these diseases. In this study, we isolated 56 marine actinomycetes, and strain AM-4 isolated from Drupa granulata exhibited the strongest antagonistic activity against P. digitatum. Molecular, morphological, cultural and physio-biochemical analysis identified strain AM-4 as Streptomyces chumphonensis. Strain AM-4 also exhibited antagonistic activity against 11 other phytopathogens, with an inhibition zone width ranging from 0.65 ± 0.04 to 2.35 ± 0.02 cm. The mycelial growth inhibition rates of the fermentation liquid of strain AM-4 against P. digitatum and P. italicum were 66.23% and 61.42%, respectively, and the conidial germination inhibition rates were 65.77% and 62.13%, respectively. The relative control efficacy of the gradient-diluted fermentation extracts of strain AM-4 against citrus green mould caused by P. digitatum ranged from 77.95% to 92.91% at 5 days post inoculation and 56.30% to 87.22% 10 days after inoculation. The relative control efficacy of the gradient diluted fermentation extracts of strain AM-4 against citrus blue mould caused by P. italicum ranged from 61.32% to 84.91% 5 days after inoculation and from 51.34% to 80.92% 10 days after inoculation. The relative control efficacy of the fermentation extracts of strain AM-4 was even better than prochloraz against citrus green mould caused by prochloraz-resistant P. digitatum. Therefore, as a biological control strain, AM-4 has a good prospect for development and utilization potential.