Phosphoenolpyruvate carboxylase of maize leaves: An improved method for purification and reduction of the inhibitory effect of malate by ethylene glycol and bicarbonate

Abstract

Light-adapted and dark-adapted forms of phosphoenolpyruvate carboxylase were purified from maize leaves by an improved method that included chromatography on Butyl-Toyopearl in the presence of ethylene glycol. The inhibition by malate was relieved not only by increasing concentrations of ethylene glycol but also by bicarbonate at pH 7.0.