Biofilm Formation by Marine Cobetia marina alex and Pseudoalteromonas spp: Development and Detection of Quorum Sensing N-Acyl Homoserine Lactones (AHLs) Molecules

Abstract

Surfaces submerged in seawater are colonized by various microorganisms, resulting in the formation of heterogenic marine biofilms. This work aims to evaluate the biofilm formation by Cobetia marina alex and doing a comparative study between this promising strain with the two bacterial strains isolated previously from the Mediterranean seawater, Alexandria, Egypt. Three strains; Cobetia marina alex, Pseudoalteromonas sp. alex, and Pseudoalteromonas prydzensis alex were screened for biofilm formation using the crystal violet (CV) quantification method in a single culture. The values of biofilm formed were OD600= 3.0, 2.7, and 2.6, respectively leading to their selection for further evaluation. However, factors affecting biofilm formation by C. marina alex were investigated. Biofilm formation was evaluated in single and multispecies consortia. Synergistic and antagonistic interactions proved in this work lead to the belief that these bacteria have the capability to produce some interesting signal molecules N-acyl Homoserine Lactones (AHLs)