Construction and application of efficient Ac-Ds transposon tagging vectors in rice

17Citations
Citations of this article
24Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Transposons are effective mutagens alternative to T-DNA for the generation of insertional mutants in many plant species including those whose transformation is inefficient. The current strategies of transposon tagging are usually slow and labor-intensive and yield low frequency of tagged lines. We have constructed a series of transposon tagging vectors based on three approaches: (i) AcTPase controlled by glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain (GVG) chemical-inducible expression system; (ii) deletion of AcTPase via Cre-lox site-specific recombination that was initially triggered by Ds excision; and (iii) suppression of early transposition events in transformed rice callus through a dual-functional hygromycin resistance gene in a novel Ds element (HPT-Ds). We tested these vectors in transgenic rice and characterized the transposition events. Our results showed that these vectors are useful resources for functional genomics of rice and other crop plants. The vectors are freely available for the community. 2009 Institute of Botany, the Chinese Academy of Sciences.

Cite

CITATION STYLE

APA

Qu, S., Jeon, J. S., Ouwerkerk, P. B. F., Bellizzi, M., Leach, J., Ronald, P., & Wang, G. L. (2009). Construction and application of efficient Ac-Ds transposon tagging vectors in rice. Journal of Integrative Plant Biology, 51(11), 982–992. https://doi.org/10.1111/j.1744-7909.2009.00870.x

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free