Genome-wide gene expression profiling of whole blood is an attractive method for discovery of biomarkers due to its non-invasiveness, simple clinical site processing and rich biological content. Except for a few successes, this technology has not yet matured enough to reach its full potential of identifying biomarkers useful for clinical prognostic and diagnostic applications or in monitoring patient response to therapeutic intervention. A variety of technical problems have hampered efforts to utilize this technology for identification of biomarkers. One significant hurdle has been the high and variable concentrations of globin transcripts in whole blood total RNA potentially resulting in non-specific probe binding and high background. In this study, we investigated and quantified the power of three whole blood profiling approaches to detect meaningful biological expression patterns.
Parrish, M. L., Wright, C., Rivers, Y., Argilla, D., Collins, H., Leeson, B., … Lejnine, S. (2010). cDNA targets improve whole blood gene expression profiling and enhance detection of pharmocodynamic biomarkers: A quantitative platform analysis. Journal of Translational Medicine, 8. https://doi.org/10.1186/1479-5876-8-87