A new immunosuppressive pregnane glycoside and two known analogues from epigynum cochinchinensis

Abstract

Phytochemical investigation of the ethyl acetate soluble fraction from the leaves of Epigynum cochinchinensis led to the isolation of one new C21 pregnane glycoside, epigycoside C (1), along with two known analogues epigycoside A (2) and epigynoside G (3). Their structures were elucidated on the basis of extensive spectroscopic techniques. The in vitro immunosuppressive activity of compound 1 was evaluated against concanavalin A (Con A)- and lipopolysaccharides (LPS)-stimulated proliferation of mice splenocytes. Compound 1 displayed significant immunosuppressive activities in a dose-dependent manner.