The H-subunit of the photosynthetic reaction center of Rhodobacter capsulatus was synthesized in vitro by programming a R. capsulatus cell-free translation system with isolated H-protein mRNA. When intracytoplasmic membrane vehicles of R. capsulatus were added cotranslationally, newly synthesized H-protein sedimented on sucrose gradients with the membranes. The majority of the membrane-associated H-protein was resistant towards extraction with 6 M urea or 0.2 M Na2CO3 at pH 10.5. The urea-extractibility, however, increased significantly when membranes had been treated with trypsin prior to the insertion of the H-subunit, suggesting that the membrane assembly of the H-protein is dependent on (a) proteinaceous factor(s). © 1992.
Troschel, D., Eckhardt, S., Hoffschulte, H. K., & Müller, M. (1992). Cell-free synthesis and membrane-integration of the reaction center subunit H from Rhodobacter capsulatus. FEMS Microbiology Letters, 91(2), 129–133. https://doi.org/10.1016/0378-1097(92)90672-B