Cell-free synthesis and membrane-integration of the reaction center subunit H from Rhodobacter capsulatus

3Citations
Citations of this article
8Readers
Mendeley users who have this article in their library.

Abstract

The H-subunit of the photosynthetic reaction center of Rhodobacter capsulatus was synthesized in vitro by programming a R. capsulatus cell-free translation system with isolated H-protein mRNA. When intracytoplasmic membrane vehicles of R. capsulatus were added cotranslationally, newly synthesized H-protein sedimented on sucrose gradients with the membranes. The majority of the membrane-associated H-protein was resistant towards extraction with 6 M urea or 0.2 M Na2CO3 at pH 10.5. The urea-extractibility, however, increased significantly when membranes had been treated with trypsin prior to the insertion of the H-subunit, suggesting that the membrane assembly of the H-protein is dependent on (a) proteinaceous factor(s). © 1992.

Cite

CITATION STYLE

APA

Troschel, D., Eckhardt, S., Hoffschulte, H. K., & Müller, M. (1992). Cell-free synthesis and membrane-integration of the reaction center subunit H from Rhodobacter capsulatus. FEMS Microbiology Letters, 91(2), 129–133. https://doi.org/10.1016/0378-1097(92)90672-B

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free