Myc promoter-binding protein-1 (MBP-1) is a shorter protein variant of the glycolytic enzyme alpha-enolase. Although several lines of evidence indicate that MBP-1 acts as a tumor suppressor, the cellular mechanisms and signaling pathways underlying MBP-1 expression still remain largely elusive. To dissect these pathways, we used the SkBr3 breast cancer cell line and non-tumorigenic HEK293T cells ectopically overexpressing alpha-enolase/MBP-1. Here, we demonstrate that induced cell stresses promote MBP-1 expression through the AKT/PERK/eIF2α signaling axis. Our results contribute to shedding light on the molecular mechanisms underlying MBP-1 expression in non-tumorigenic and cancer cells.
Maranto, C., Perconti, G., Contino, F., Rubino, P., Feo, S., & Giallongo, A. (2015). Cellular stress induces cap-independent alpha-enolase/MBP-1 translation. FEBS Letters, 589(16), 2110–2116. https://doi.org/10.1016/j.febslet.2015.06.030