Effects of chlorpyrifos-methyl, chlormequat, deltamethrin, glyphosate, pirimiphos-methyl, tebuconazole and their mixture on oxidative stress and toxicity in HUVEC cell line

Abstract

Background and Aims: Humans and animals have daily contact with various chemicals, including food additives, pesticides, antibiotics, other veterinary drugs, and other xenobiotics. Pesticide exposure causes many health disorders. Mixed exposure to pesticides is an important issue for human and environmental health. Methods: In this study, we have determined the cytotoxicity of chlormequat pirimiphos-methyl, glyphosate, tebuconazole, chlorpyrifos-methyl, deltamethrin, and the mixture of these six pesticides. We further investigated the role of oxidative stress, total oxidant status (TOS), lactate dehydrogenase (LDH) and antioxidant defense mechanism TOS, total glutathione (GSH) levels with the observed cytotoxicity. Results: In this study, the mixtures of pesticides reduced total antioxidant status (TAS) and GSH level one by one and increased the reactive oxygen species (ROS) generation in HUVECs, respectively. The results also showed a significant contribution of oxidative stress on cytotoxicity during pesticide mixture exposure. Conclusion: The findings are that pesticide mixture exposure might have an impact on human health risk at contaminated sites and under occupational exposure conditions.