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Virus-induced gene silencing in diploid and tetraploid potato species

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Abstract

Potato is the world’s fourth largest food crop and a vegetatively propagated model polyploid plant. To facilitate genomic studies in potato, here we describe detailed protocols to silence genes in both diploid potato Solanum bulbocastanum and tetraploid potato cultivars such as Maris Bard, Arran Pilot, Ancilla, and Serrana using tobacco rattle virus (TRV)- or potato virus X (PVX)-induced gene silencing (VIGS) system, respectively. The established VIGS system represents an efficient and powerful approach for functional analysis of genes involved in growth, development, metabolism, and responses to biotic and abiotic stresses in potato.

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Zhao, J., Jiang, H., Wang, G., Wang, Z., Dong, J., & Song, J. (2020). Virus-induced gene silencing in diploid and tetraploid potato species. In Methods in Molecular Biology (Vol. 2172, pp. 39–50). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0751-0_4

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