Characterization of BshA, bacillithiol glycosyltransferase from Staphylococcus aureus and Bacillus subtilis

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Abstract

The first step during bacillithiol (BSH) biosynthesis involves the formation of N-acetylglucosaminylmalate from UDP-N-acetylglucosamine and l-malate and is catalyzed by a GT4 class glycosyltransferase enzyme (BshA). Recombinant Staphylococcus aureus and Bacillus subtilis BshA were highly specific and active with l-malate but the former showed low activity with d-glyceric acid and the latter with d-malate. We show that BshA is inhibited by BSH and similarly that MshA (first enzyme of mycothiol biosynthesis) is inhibited by the final product MSH. © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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Upton, H., Newton, G. L., Gushiken, M., Lo, K., Holden, D., Fahey, R. C., & Rawat, M. (2012). Characterization of BshA, bacillithiol glycosyltransferase from Staphylococcus aureus and Bacillus subtilis. FEBS Letters, 586(7), 1004–1008. https://doi.org/10.1016/j.febslet.2012.02.028

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