Both Reelin and Nerve Growth Factor (NGF) exert crucial roles in retinal development. Retinogenesis is severely impaired in E-reeler mice, a model of Reelin deficiency showing specific Green Fluorescent Protein expression in Rod Bipolar Cells (RBCs). Since no data are available on Reelin and NGF cross-talk, NGF and trkANGFR / p75NTR expression was investigated in retinas from E-reeler versus control mice, by confocal microscopy, Western blotting, and real time PCR analysis. A scattered increase of NGF protein was observed in the Ganglion Cell Layer and more pronounced in the Inner Nuclear Layer (INL). A selective increase of p75NTR was detected in most of RBCs and in other cell subtypes of INL. On the contrary, a slight trend towards a decrease was detected for trkANGFR , albeit not significant. Confocal data were validated by Western blot and real time PCR. Finally, the decreased trkANGFR / p75NTR ratio, representative of p75NTR increase, significantly correlated with E-reeler versus E-control. These data indicate that NGF- trkANGFR / p75NTR is affected in E-reeler retina and that p75NTR might represent the main NGF receptor involved in the process. This first NGF- trkANGFR / p75NTR characterization suggests that E-reeler might be suitable for exploring Reelin-NGF cross-talk, representing an additional information source in those pathologies characterized by retinal degeneration.
Balzamino, B. O., Biamonte, F., Esposito, G., Marino, R., Fanelli, F., Keller, F., & Micera, A. (2014). Characterization of NGF, trkA NGFR , and p75 NTR in Retina of Mice Lacking Reelin Glycoprotein . International Journal of Cell Biology, 2014, 1–13. https://doi.org/10.1155/2014/725928