Bordetella hinzii has recently been isolated from immunocompromised human hosts. The polysaccharides isolated from its endotoxin (lipopolysaccharide, LPS) were investigated using chemical analyses, NMR, gas-liquid chromatography/mass spectrometry and mass spectrometry by plasma desorption, matrix-assisted laser desorption/ionization and electrospray. The following structure for the O-chain-free LPS was deduced from the experimental results: Mass spectrometry and serology revealed that the O-chains were different from the homopolymer common to Bordetella bronchiseptica and Bordetella parapertussis strains and were composed of a trisaccharide repeating unit. Masses up to 8 kDa were obtained for native LPS molecular species. (C) 2000 Federation of European Biochemical Societies.
Aussel, L., Chaby, R., Le Blay, K., Kelly, J., Thibault, P., Perry, M. B., & Caroff, M. (2000). Chemical and serological characterization of the Bordetella hinzii lipopolysaccharides. FEBS Letters, 485(1), 40–46. https://doi.org/10.1016/S0014-5793(00)02181-5