CK1  and CK1  are components of human 40S subunit precursors required for cytoplasmic 40S maturation

  • Zemp I
  • Wandrey F
  • Rao S
  • et al.
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Abstract

Biogenesis of 40S pre-ribosomal subunits requires many transacting factors, among them several protein kinases. In this study, we show that the human casein kinase 1 (CK1) isoforms δ and ε are required for cytoplasmic maturation steps of 40S subunit precursors. We show that both CK1δ and CK1ε isoforms are components of pre-40S subunits, on which they phosphorylate the ribosome biogenesis factors ENP1/BYSL and LTV1. Inhibition or codepletion of CK1δ and CK1ε results in failure to recycle a series of trans-acting factors including ENP1/BYSL, LTV1, RRP12, DIM2/ PNO1, RIO2 and NOB1 from pre-40S particles after nuclear export. Furthermore, co-depletion of CK1δ and CK1ε leads to defects in 18S-E pre-rRNA processing. Together, these data demonstrate that CK1δ and CK1ε play a decisive role in triggering late steps of pre- 40S maturation that are required for acquisition of functionality of 40S ribosomal subunits in protein translation. © 2014. Published by The Company of Biologists Ltd.

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APA

Zemp, I., Wandrey, F., Rao, S., Ashiono, C., Wyler, E., Montellese, C., & Kutay, U. (2014). CK1  and CK1  are components of human 40S subunit precursors required for cytoplasmic 40S maturation. Journal of Cell Science, 127(6), 1242–1253. https://doi.org/10.1242/jcs.138719

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