CLAVATA3 dodecapeptide modified CdTe nanoparticles: A biocompatible quantum dot probe for in vivo labeling of plant stem cells

3Citations
Citations of this article
18Readers
Mendeley users who have this article in their library.

Abstract

CLAVATA3 (CLV3) dodecapeptides function in plant stem cell maintenance, but CLV3 function in cell-cell communication remains less clear. Here, we coupled CLV3 dodecapeptides to synthesized CdTe nanoparticles to track their bioactivity on stem cells in the root apical meristem. To achieve this, we first synthesized CdTe quantum dots (QDs) using a one-pot method, and then evaluated the cytotoxicity of the QDs in BY-2 cells. The results showed that QDs in plant cells must be used at low concentrations and for short treatment time. To make biocompatible probes to track stem cell fate, we conjugated CLV3 dodecapeptides to the QDs by the zero-coupling method; this modification greatly reduced the cytotoxicity of the QDs. Furthermore, we detected CLV3-QDs localized on the cell membrane, consistent with the known localization of CLV3. Our results indicate that using surface-modified QDs at low concentrations and for short time treatment can improve their utility for plant cell imaging. © 2014 Yu et al.

Cite

CITATION STYLE

APA

Yu, G., Tan, Y., He, X., Qin, Y., & Liang, J. (2014). CLAVATA3 dodecapeptide modified CdTe nanoparticles: A biocompatible quantum dot probe for in vivo labeling of plant stem cells. PLoS ONE, 9(2). https://doi.org/10.1371/journal.pone.0089241

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free