Cloning of a melatonin-related receptor from human pituitary

125Citations
Citations of this article
44Readers
Mendeley users who have this article in their library.

Abstract

We have cloned an orphan G protein-coupled receptor from a human pituitary cDNA library using a probe generated by PCR. The cDNA, designated H9, encodes a protein of 613 amino acids that is 45% identical at the amino acid level to the recently cloned human Mel(1a) and Mel(1b) melatonin receptors. Structural analyses of the encoded protein and its gene, along with phylogenetic analysis, further show that H9 is closely related to the G protein-coupled melatonin receptor family. Unusual features of the protein encoded by H9 include a lack of N-linked glycosylation sites and a carboxyl tail >300 amino acids long. H9 transiently expressed in COS-1 cells did not bind [125I]melatonin or [3H]melatonin. H9 mRNA is expressed in hypothalamus and pituitary, suggesting that the encoded receptor and its natural ligand are involved in neuroendocrine function.

Cite

CITATION STYLE

APA

Reppert, S. M., Weaver, D. R., Ebisawa, T., Mahle, C. D., & Kolakowski, L. F. (1996). Cloning of a melatonin-related receptor from human pituitary. FEBS Letters, 386(2–3), 219–224. https://doi.org/10.1016/0014-5793(96)00437-1

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free