Cloning and sequencing of rat liver cDNAs encoding the regulatory protein of glucokinase

29Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.

Abstract

cDNAs encoding the rat liver regulatory protein of glucokinase were cloned and sequenced. The deduced protein contains 568 amino acids for a molecular mass of 62,867 Da. Northern blot analysis showed the presence of a major RNA species of 2.35 kb in rat liver. No signal was observed with muscle, brain, heart, testis, intestine or spleen RNA. Recombinant regulatory protein expressed in Escherichia coli was insoluble and inactive, and was presumably contained in inclusion bodies. Western blot analysis showed that the recombinant protein was recognized by antibodies raised against regulatory protein purified from rat liver. © 1993.

Cite

CITATION STYLE

APA

Detheux, M., Vandekerckhove, J., & Van Schaftingen, E. (1993). Cloning and sequencing of rat liver cDNAs encoding the regulatory protein of glucokinase. FEBS Letters, 321(2–3), 111–115. https://doi.org/10.1016/0014-5793(93)80089-D

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free