Clostridium perfringens iota toxin ADP-ribosylates skeletal muscle actin in Arg-177

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Abstract

Clostridium perfringens iota toxin ADP-ribosylates actin. Substrates of C. perfringens toxin are both non-muscle β/γ-actin and skeletal muscle actin. This finding suggests that C. perfringens iota ADP-ribosylates the same amino acid in skeletal muscle and non-muscle actin as does C. botulinum C2 toxin in non-muscle actin. Protein chemical analysis involving thermolysin cleavage on [32P]ADP-ribosylated actin or tryptic digestion followed by a secondary thermolysin cleavage of the radiolabelled fragments showed one major site of ADP-ribosylation. From its amino acid composition and sequence, the radiolabelled peptide was identified as peptide 175-177, locating the acceptor ADP-ribosyl amino acid as Arg-177. © 1987.

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Vandekerckhove, J., Schering, B., Bärmann, M., & Aktories, K. (1987). Clostridium perfringens iota toxin ADP-ribosylates skeletal muscle actin in Arg-177. FEBS Letters, 225(1–2), 48–52. https://doi.org/10.1016/0014-5793(87)81129-8

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