Objective . To develop and compare HPLC-DAD and UHPLC-UV assays for the quantitation of posaconazole in bulk powder and suspension dosage form. Methods . Posaconazole linearity range was 5–50 μ g/mL for both assays. For HPLC-DAD assay, samples were injected through Zorbax SB-C18 (4.6 × 250 mm, 5 μ m) column. The gradient elution composed of the mobile phase acetonitrile: 15 mM potassium dihydrogen orthophosphate (30 : 70 to 80 : 20, linear over 7 minutes) pumped at 1.5 mL/min. For UHPLC-UV assay, samples were injected through Kinetex-C18 (2.1 × 50 mm, 1.3 μ m) column. The mobile phase composed of acetonitrile: 15 mM potassium dihydrogen orthophosphate (45 : 55) pumped isocratically at 0.4 mL/min. Detection wavelength was 262 nm in both methods. Results . The run time was 11 and 3 minutes for HPLC-DAD and UHPLC-UV assays, respectively. Both assays were linear ( r2>0.999 ) with CV% and % error of the mean <3%. Limits of detection and quantitation were 0.82 and 2.73 μ g/mL for HPLC-DAD and 1.04 and 3.16 μ g/mL for UHPLC-UV, respectively. The methods quantitated PSZ in suspension dosage form with no observable interferences. Conclusions . Both assays were proven sensitive and selective according to ICH guidelines. UHPLC-UV assay exhibited some economic and chromatographic separation superiority.
Hamdy, D. A., & Belal, T. S. (2014). A comparative study of newly developed HPLC-DAD and UHPLC-UV assays for the determination of posaconazole in bulk powder and suspension dosage form. Journal of Analytical Methods in Chemistry, 2014. https://doi.org/10.1155/2014/241035