Comparison of oriented and random antibody immobilization techniques on the efficiency of immunosensor

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Abstract

An immunosensor for bovine leukemia virus antigen (gp51) determination was developed using surface plasmon resonance (SPR) technique. During this work the SPR-chip coated with gold was used for immunosensor design. Different strategies of antibodies immobilization - oriented immobilization of specific antibodies fragments (fraganti- gp51) on gold surface obtained after chemical reduction and random immobilization of intact antibodies (antigp51) on gold surface, were investigated. The immobilization based on the application of frag-anti-gp51 was found to be the most suitable for the design of SPR-immunosensor for gp51 detection. SPR sensor created using frag-antigp51 was tested in a range from 0.01 to 0.5 mg/mL of gp51 antigen concentration. Developed immunosensor exhibited sufficient antigen binding capacity, simplicity and low cost in respect to the currently evaluated techniques. © 2012 The Authors. Published by Elsevier Ltd.

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Baniukevic, J., Kirlyte, J., Ramanavicius, A., & Ramanaviciene, A. (2012). Comparison of oriented and random antibody immobilization techniques on the efficiency of immunosensor. In Procedia Engineering (Vol. 47, pp. 837–840). Elsevier Ltd. https://doi.org/10.1016/j.proeng.2012.09.277

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