Complete genome sequencing of Pandoraea pnomenusa RB38 and Molecular Characterization of Its N -acyl homoserine lactone synthase gene ppnI

  • Lim Y
  • Ee R
  • How K
  • et al.
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Abstract

© 2015 Lim et al. In this study, we sequenced the genome of Pandoraea pnomenusa RB38 using Pacific Biosciences RSII (PacBio) Single Molecule Real Time (SMRT) sequencing technology. A pair of cognate luxI/R homologs was identified where the luxI homolog, ppnI, was found adjacent to a luxR homolog, ppnR1. An additional orphan luxR homolog, ppnR2, was also discovered. Multiple sequence alignment and phylogenetic analysis revealed that ppnI is an N-acyl homoserine lactone (AHL) synthase gene that is distinct fromthose of the nearest phylogenetic neighbor viz. Burkholderia spp. High resolution tandem mass spectrometry (LC-MS/MS) analysis showed that Escherichia coli BL21 harboring ppnI produced a similar AHL profile (N-octanoylhomoserine lactone, C8-HSL) as P. pnomenusa RB38, the wild-type donor strain, confirming that PpnI directed the synthesis of AHL in P. pnomenusa RB38. To our knowledge, this is the first documentation of the luxI/R homologs of the genus Pandoraea.

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APA

Lim, Y.-L., Ee, R., How, K.-Y., Lee, S.-K., Yong, D., Tee, K. K., … Chan, K.-G. (2015). Complete genome sequencing of Pandoraea pnomenusa RB38 and Molecular Characterization of Its N -acyl homoserine lactone synthase gene ppnI . PeerJ, 3, e1225. https://doi.org/10.7717/peerj.1225

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