The archaeal parasite Nanoarchaeum equitans was found to generate five tRNA species via a unique process requiring the assembly of seperate 5′ and 3′ tRNA halves [Randau, L., Münch, R., Hohn, M.J., Jahn, D. and Söll, D. (2005) Nanoarchaeum equitans creates functional tRNAs from separate genes for their 5′- and 3′-halves. Nature 433, 537-541]. Biochemical evidence was missing for one of the computationally-predicted, joined tRNAs designated as tRNA Trp. Our RT-PCR and sequencing results identify this tRNA as tRNA Lys (CUU) joined at the alternative position between bases 30 and 31. We show that the intron-containing tRNA Trp was misidentified in the initial Nanoarchaeum equitans genome annotation [E. Waters et al. (2003) The genome of Nanoarchaeum equitans: insights into early archaeal evolution and derived parasitism. Proc. Natl. Acad. Sci. USA 100, 12984-12988]. Along with a previously unidentified joined tRNA Gln (UUG), Nanoarchaeum equitans exhibits 44 tRNAs and is enabled to read all 61 sense codons. Features unique to this set of tRNA molecules are discussed. © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Randau, L., Pearson, M., & Söll, D. (2005). The complete set of tRNA species in Nanoarchaeum equitans. FEBS Letters, 579(13), 2945–2947. https://doi.org/10.1016/j.febslet.2005.04.051