Conservation of a novel protein associated with an antibiotic efflux operon in Burkholderia cenocepacia

12Citations
Citations of this article
15Readers
Mendeley users who have this article in their library.

Abstract

Burkholderia cenocepacia is a significant problem in individuals with cystic fibrosis and is a member of the B. cepacia complex of closely related antibiotic resistant bacteria. A salicylate-regulated antibiotic efflux operon has been identified in B. cenocepacia and one of its four genes, llpE, is without parallel in previously reported efflux operons. PCR amplification and sequencing of llpE from B. cepacia complex isolates demonstrated the highest prevalence in B. cenocepacia with a high degree of sequence conservation. While at least one non-synonymous mutation was identified between isolates from different genomovars, only synonymous differences were identified within the IIIA and IIIB sub-groups of B. cenocepacia. Structural modeling suggests that LlpE is a member of the α/β hydrolase enzyme family. Identification of strong structural homology to hydrolases and a high degree of conservation in B. cenocepacia suggests an enzymatic function for LlpE, benefiting survival in the cystic fibrosis lung. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Cite

CITATION STYLE

APA

Nair, B. M., Joachimiak, L. A., Chattopadhyay, S., Montano, I., & Burns, J. L. (2005). Conservation of a novel protein associated with an antibiotic efflux operon in Burkholderia cenocepacia. FEMS Microbiology Letters, 245(2), 337–344. https://doi.org/10.1016/j.femsle.2005.03.027

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free