Hepatic stimulator substance (HSS) protects liver cells from various toxins by alleviating lesions caused in the mitochondria. This paper demonstrates the necessity of the conserved CXXC catalytic motif (C62-C65) for the mitochondria-targeted anti-apoptotic activity of HSS. Mutating the conserved CXXC motif eliminated the protective effects against H 2 O 2 -induced apoptosis and diminished the protection of the mitochondria. However, the mutation of the other disulfide bond C91-C108 mainly preserved the protection of mitochondria by HSS, implying that the conserved CXXC motif and sulfhydryl oxidase (SOX) activity are essential for mitochondrial protection. Structured summary: MINT- 7992635: HSS (uniprotkb:. P55789) and HSS (uniprotkb:. P55789) bind (MI:. 0407) by chromatography technology (MI:. 0091). © 2010 Federation of European Biochemical Societies.
Li, W., Zhang, J., & An, W. (2010). The conserved CXXC motif of hepatic stimulator substance is essential for its role in mitochondrial protection in H 2 O 2 -induced cell apoptosis. FEBS Letters, 584(18), 3929–3935. https://doi.org/10.1016/j.febslet.2010.08.043