Metalloprotein properties result from the interplay between coordination requirements of the metal center, protein stability, and modulation of the metal center by the surrounding protein matrix. Simple metal centers, which exercise control over the protein by affecting stability or enzyme activity, have been created by rational design. Complex centers, which require control by the protein matrix, have also been constructed.
Hellinga, H. W. (1998). The construction of metal centers in proteins by rational design. Folding and Design, 3(1). https://doi.org/10.1016/S1359-0278(98)00001-7