Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: Proof of principle

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Abstract

Selenium was incorporated into an oligodeoxynucleotide in the form of 2′-methylseleno-uridine (USe). The X-ray crystal structure of the duplex [d(GCGTA)USed(ACGC)]2 was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 Å, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2′-α-position with selenium does not affect the preference of the sugar for the C3′-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidins. © 2002 Société française de biochimie et biologie moléculaire / Éditions scientifiques et médicales Elsevier SAS. All rights reserved.

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Teplova, M., Wilds, C. J., Wawrzak, Z., Tereshko, V., Du, Q., Carrasco, N., … Egli, M. (2002). Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: Proof of principle. Biochimie, 84(9), 849–858. https://doi.org/10.1016/S0300-9084(02)01440-2

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