The Cryoelectron Microscopy Structure of the Type 1 Chaperone-Usher Pilus Rod

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Abstract

Adhesive chaperone-usher pili are long, supramolecular protein fibers displayed on the surface of many bacterial pathogens. The type 1 and P pili of uropathogenic Escherichia coli (UPEC) play important roles during urinary tract colonization, mediating attachment to the bladder and kidney, respectively. The biomechanical properties of the helical pilus rods allow them to reversibly uncoil in response to flow-induced forces, allowing UPEC to retain a foothold in the unique and hostile environment of the urinary tract. Here we provide the 4.2-Å resolution cryo-EM structure of the type 1 pilus rod, which together with the previous P pilus rod structure rationalizes the remarkable “spring-like” properties of chaperone-usher pili. The cryo-EM structure of the type 1 pilus rod differs in its helical parameters from the structure determined previously by a hybrid approach. We provide evidence that these structural differences originate from different quaternary structures of pili assembled in vivo and in vitro. Chaperone-usher pili are crucial virulence factors of uropathogenic E. coli that mediate host recognition and adhesion. Hospenthal et al. present the cryo-EM structure of the type 1 pilus rod explaining its “spring-like” biomechanical properties. Biochemical data suggest that the rod can exist in more than one helical quaternary assembly.

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Hospenthal, M. K., Zyla, D., Costa, T. R. D., Redzej, A., Giese, C., Lillington, J., … Waksman, G. (2017). The Cryoelectron Microscopy Structure of the Type 1 Chaperone-Usher Pilus Rod. Structure, 25(12), 1829-1838.e4. https://doi.org/10.1016/j.str.2017.10.004

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