Crystallization and preliminary X-ray analysis of porin from Rhodobacter capsulatus

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Abstract

Porin monomers of the phototrophic bacterium Rhodobacter capsulatus were purified. Crystals were obtained from a solution of porin solubilized with the detergent octyltetraoxyethylene within 5 days using the vapor phase equilibration technique. The crystals were rhombohedral with an edge length of 0.4 mm. The space group was trigonal R3 with unit cell constants of a=b=95 Å, c=147 Å. Reflexions were observed to 3.2 Å. © 1989.

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Nestel, U., Wacker, T., Woitzik, D., Weckesser, J., Kreutz, W., & Welte, W. (1989). Crystallization and preliminary X-ray analysis of porin from Rhodobacter capsulatus. FEBS Letters, 242(2), 405–408. https://doi.org/10.1016/0014-5793(89)80511-3

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