Chitosan-Polyacrylic Acid Hybrid Nanoparticles as Novel Tissue Adhesive: Synthesis and Characterization

Abstract

Chitosan (Cs)-poly acrylic acid (PAA) complex nanoparticles well dispersed and stable in aqueous solution was prepared by free radical polymerization of acrylic acid monomers (AA) in the presence of chitosan (Cs). Different concentrations of Cs and AA was cross-linked to form scalable hybrid nanoparticles (HNP). The physicochemical characterization of nanopolymers was investigated using FTIR, dynamic light scattering (DLS) and scanning electron microscopy (SEM). Infrared spectroscopy and electronic microscopy analysis results showed the preparation of Cs-PAA hybrid nanoparticles due to the formation of polyelectrolyte complexes and their spherical shape, respectively. The average particle size obtained under 180 nm for all samples obtained by using DLS analysis. A dhesion strength for the samples with a ratio of Cs/AA 1: 1 and 0.2: 1 was 50 to 120 kPa and 120 to 230 kPa, respectively. Cytotoxicity assay (MTT) showed that cell activity for concentrations X/5 and X/10 was over 7 5 %, demonstrating the sample extracts have suitable cytocompatibility. In addition, hemolytic assay of the samples represents below 0.5 % (according to the standard) in damage extend to red blood cells. Furthermore, prepared tissue adhesive based on Cs-PAA hybrid NPs in terms of adhesion strength was competitive with commercial samples as well as satisfactory results in terms of biocompatibility and blood compatibility were achieved in comparison with commercial available adhesives based on cyanoacrylate.