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Analysis of chromatin interactions mediated by specific architectural proteins in drosophila cells

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Abstract

Chromosome conformation capture assays have been established, modified, and enhanced for over a decade with the purpose of studying nuclear organization. A recently published method uses in situ Hi-C followed by chromatin immunoprecipitation (HiChIP) to enrich the overall yield of significant genome-wide interactions mediated by a specific protein. Here we applied a modified version of the HiChIP protocol to retrieve the significant contacts mediated by architectural protein CP190 in D. melanogaster cells.

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Ando-Kuri, M., Rivera, I. S. M., Rowley, M. J., & Corces, V. G. (2018). Analysis of chromatin interactions mediated by specific architectural proteins in drosophila cells. In Methods in Molecular Biology (Vol. 1766, pp. 239–256). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7768-0_14

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