The effect of re-oxidation on the reduced hydrogenase of Desulfovibrio vulgaris strain Hildenborough and its oxygen stability

Abstract

Upon reduction of the oxygen-stable hydrogenase of Desulfovibrio vulgaris strain Hildenborough this enzyme becomes oxygen sensitive. Only anaerobic oxidation of the reduced enzyme with 2,6-dichlorophenol-indophenol, in the presence of iron and EDTA, followed by addition of Tris-EDTA (pH 9.0) converts the enzyme back into its oxygen-stable state, without loss of activity. © 1983.