The effect of re-oxidation on the reduced hydrogenase of Desulfovibrio vulgaris strain Hildenborough and its oxygen stability

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Abstract

Upon reduction of the oxygen-stable hydrogenase of Desulfovibrio vulgaris strain Hildenborough this enzyme becomes oxygen sensitive. Only anaerobic oxidation of the reduced enzyme with 2,6-dichlorophenol-indophenol, in the presence of iron and EDTA, followed by addition of Tris-EDTA (pH 9.0) converts the enzyme back into its oxygen-stable state, without loss of activity. © 1983.

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APA

Dijk, C. van, Berkel-Arts, A. van, & Veeger, C. (1983). The effect of re-oxidation on the reduced hydrogenase of Desulfovibrio vulgaris strain Hildenborough and its oxygen stability. FEBS Letters, 156(2), 340–344. https://doi.org/10.1016/0014-5793(83)80525-0

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