Spermatozoa motility and short-term sperm storage of colourful orfe (Leuciscus idus aberr orfus)

Abstract

In this study the effect of six activating buffers on the movement parameters of sperm were determined and short-term storage of semen in TLP buffer was attempted (0.292 g NaCl; 0.012g KCl; 0.011g CaCl 2; 0.004g MgCl 2; 0.105g NaHCO 3; 0.002g NaH 2PO 4; 50 mL; pH 8.6). Sperm was collected from five orfe individual, and spermiation was stimulated by means of an intraperitoneal Ovopel injection. The basic parameters of spermatozoa motility were measured after the semen was diluted with six different activating solutions, previously used successfully in other fish species. The motility analysis was conducted on a Crismas apparatus. Additionally, short-term storage of semen in TLP buffer was attempted. Subsequently, motility parameters were verified after 0 (Control), 24 and 120 h of storage at 4°C. It has been found that Lahnsteiner's buffer (100 mM NaCl, 10 mM Tris, 0.5% albumin, 199 mOsmkg -1) was found to be the most effective in sperm activation. In this paper, the spermatozoa motility of colourful ide is indicated for the first time. Finally, there was asuccessful attempt at short-term sperm storage for five days. For artificial insemination, itis very important to select the most effectivesolution to stimulate sperm motility. Data regarding sperm manipulation of orfe are scarce, so the aim of the study was to determine the basic sperm quality parameters of the colour ide form, i.e. Leuciscus idus aberr orfus. © B. Sarosiek et al., 2012.